Kemtec Science: Physical Properties of Proteins

Kemtec Science: Physical Properties of Proteins

• Target Grades: 9-14
• Number of Students: 24 students working in six groups of four

This kit demonstrates the important effects of physical parameters such as temperature, pH, salt concentration, and dielectric constants on proteins and illustrates how these effects are used in the isolation and purification of proteins. A protein amino acid composition and structure influence its physical characteristics. Experiments include: Isoelectric Precipitation of Casein, Separation of Albumin and Gelatin by Ammonium Sulfate Fractionation, Effects of Acetone on Albumin and Gelatin Solutions, Heat Denaturation of Albumin, and Precipitation of Protein by Lead Acetate. Contains lead acetate.

Kit Contains:

• Instructor Manual
  
• Student Manual
  
• Student Data Sheet
  
• MSDS
  
• Pipettes
  
• Empty Plastic Bottles
  
• Ammonium Sulfate
  
• Acetone Solution
  
• Glacial Acetic Acid Solution
  
• Lead Acetate Solution
  
• Sodium Hydroxide
  
• Albumin
  
• Caseine
  
• Gelatin

Required, But Not Included:

• Bunsen Burner
  
• Glassware
  
• Test Tube holders