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The enzyme Invertase hydrolyzes sucrose into glucose and fructose. The amount of these reducing sugars can be measured with the chemical DNS which creates a distinctive color change. Students use a spectrophotometer to determine the reaction rate of Invertase when subjected to varied substrate concentrations, competitive and non-competitive inhibitors, changes in pH, and changes in temperature. Kit includes all necessary chemicals and a reply card for the Invertase enzyme and glucose solutions to be shipped separately upon request. ‚ Reply card items are only shipped within the continental United States. Required, but not included: lab glassware; including beakers, test tubes, and graduated cylinders; a hot plate, and a spectrophotometer.