Kemtec Science: Enzymology, Advanced Kit

• Target Grades: 11-14
• Number of Students: 24 students working in six groups of four

The enzyme Invertase hydrolyzes sucrose into glucose and fructose. The amount of these reducing sugars can be measured with the chemical DNS which creates a distinctive color change. Students use a spectrophotometer to determine the reaction rate of Invertase when subjected to varied substrate concentrations, competitive and non-competitive inhibitors, changes in pH, and changes in temperature. Kit includes all necessary chemicals and a reply card for the Invertase enzyme and glucose solutions to be shipped separately upon request. ‚ Reply card items are only shipped within the continental United States. Required, but not included: lab glassware; including beakers, test tubes, and graduated cylinders; a hot plate, and a spectrophotometer.

Kit Contains:

• Instructor Manual
  
• Student Data Sheets
  
• MSDS
  
• Buffer pH 3 Solution
  
• Buffer pH 4 Solution
  
• Buffer pH 5 Solution
  
• Buffer pH 6 Solution
  
• Buffer pH 7 Solution
  
• Buffer pH 8 Solution
  
• Buffer pH 9 Solution
  
• Buffer pH 10 Solution
  
• Buffer Substrate Effects Solution
  
• Buffer Temperature Effects Solution
  
• Buffer Inhibition Solution
  
• Inhibitor Solution
  
• Sucrose Inhibitor
  
• Sucrose Substrate Effecis
  
• Sucrose Temperature Effects
  
• Sucrose pH Substrate
  
• 3,5 Dinitrosalicylic Solution (DNS)
  
• Aniline Solution
  
• Invertase Solution
  
• Standard Glucose Solution
  

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