Kemtec Science: Enzymes Introduction

• Target Grades: 11-14
• Number of Students: 24 students working in six groups of four.

Enzymes are sometimes present in such small amounts that the amount of enzyme is determined by measuring its “activity." This kit is designed to study the enzyme activity of Invertase, Amylase, and Pepsin using specific enzyme assay systems. It includes five introductory experiments including three for enzyme activity, one for the presence of Amylase in saliva, and one on the effect of time on enzyme activity. Invertase and Amylase activity are measured using systems with the chemical DNS which creates a distinctive color change. Students may use visual examination or a spectrophotometer to determine reaction rates. This kit includes all necessary chemicals and a reply card for Invertase, Amylase, Pepsin & Hemoglobin to be shipped separately upon request. Reply card items are only shipped within the continental United States.

Kit Contains:

• Instructor Manual‚ 
  
• Student Data Sheets
  
• Assessment Manual
  
• MSDS
  
• HCI
  
• Buffer 1
  
• Buffer 2
  
• Universal Indicator Solution
  
• Sucrose
  
• Starch
  
• DNS Reagent
  
• Syringe
  
• Reply Card for Amylase, Invertase, Pepsin, Hemoglobin

Required, But Not Included:

• Lab Glassware, Including Beakers
  
• Test Tubes
  
• Gaduated Cylinders
  
• A Hot Plate or Water Bath
  
• Spectrophotometer or Colorimeter - absorption 540 nm (optional)