Lab-Aids: DNA Staining Kit
This highly engaging Lab-Aid is based on the Feulgen reaction where DNA in plant cells are stained purple for easy viewing. Students macerate, stain, and mount specially grown root tips (provided in the kit). Then, they locate and diagram DNA in the cells. Finally, the students are asked to observe and draw the various stages of cell division. This enables the students to develop a better understanding of the hereditary material in plant chromosomes. All aspects of the lab activity are performed by the individual student. MICROSCOPES ARE NEEDED.
Scientific Concepts:
- Macerate, stain and mount selected root tips
- Locate and diagram DNA in cells
- Observe and draw various stages of cell division
Content List:
- 1 Teacher's Guide with MSDS
- 30 Student Worksheets and Guides
- 30 Microscope slides
- 30 Cover slips
- 30 Plastic treating dishes
- 30 Transfer brushes
- 1 Specially selected root tips, Vial
- 1 Dilute Acetic Acid, Drop control bottle
- 2 Hydrochloric Acid, 1 M, Drop control bottle
- 2 Leucofuchsin Dye, Drop control bottle
- 2 Sodium Bisulfite Solution, Drop control bottle
Classroom Planning:
- To complete this kit requires one ~50-minute class periods
- Number of students: 30
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