Kemtec Science: Gel Filtration, Plant Pigment

Kemtec Science: Gel Filtration, Plant Pigment

• Target Grades: 10-14
• Number of Students: 24 students working in six groups of four

This gel filtration technique allows students to take advantage of differences in molecular size and structure to separate plant pigments using chromatographic columns. Students pack their own Sephadex G-25 chromatographic columns. They observe the separation of anthocyanin, carotene, chlorophyll and xanthophyll from a mixture of these pigments. This separation is due to differences in size as well as the presence of aromatic and cyclic compounds. The greater the number of rings in the pigments, the greater the affinity of the pigments for the gel granules. Columns may be re-used.‚ 

Kit Contains:

• Instructor Manual
  
• Student Manual
  
• MSDS
  
• Student Data Sheets
  
• Ethyl Alcohol Solution
  
• Sephadex G-25
  
• Filter Paper
  
• PD-10 Columns

Required, But Not Included:

• Beet
  
• Spinach
  
• Coleus Leaves
  
• Balance
  
• Lab Glassware and Supplies