Lab-Aids: Basic Chromatography Kit

• The separation of chlorophyll extract into its constituent pigments‚ 
• The separation of dyes with polar and non-polar solvents demonstrating how chromatography is used to determine the makeup of various organic and inorganic substances.

• Understand how chromatography can be used to identify the makeup of organic and inorganic substances
• Perform chromatography to identify the constituent pigments of chlorophyll
• Observe differences in polar and non-polar solvents with dyes

• 1 Teacher's Guide with MSDS
• 30 Chromatography tubes and holders
• 120 Chromatography strips, Pre-cut paper
• 1 Developer I (petroleum ether and acetone), Bottle
• 1 Developer II (deionized water), Bottle
• 1 Developer III (n-butanol), Bottle
• 1 Developer IV (n-butanol, acetic acid and deionized water), Bottle
• 1 Capillary applicators, Vial
• 1 Chloroplast Extract, concentrated, Vial
• 1 Dyes, mixed, Vial

• To complete this kit requires 1 ~50-minute class period.
• Number of Students: 30 (if working individually, more if grouped)