Lab-Aids: DNA Fingerprinting Diagnostics (Replication, PCR and RFLP) Kit
This timely Lab-Aid introduces biotechnology, forensic science, or AP Biology students to the concepts of DNA replication and how it relates to PCR (Polymerase Chain Reaction) and RFLP (Restriction Fragment Length Polymorphism) analyses of DNA. They also observe how DNA analyses can be used to diagnose disease. This activity is a great introduction or review if you are fortunate enough to have the equipment and materials to do gel electrophoresis and DNA fingerprinting. Material in this kit, for 30 students, is reusable.
Scientific Concepts:
Construct specific genes with the characteristic nucleotide configuration for cystic fibrosis
Observe the difference between normal and mutant genes
Understand how the disease can be diagnosed by two methods of DNA analysis
Demonstrates amplification and PCR ( Polymerase Chain Reaction)
Simulate gel electrophoresis
Content List:
1Teacher's Guide
20Student Worksheets and Guides
10Nucleotides sheet: cytosine 28 (blue)
10Nucleotides sheet: guanine 28 (yellow)
10Nucleotides sheet: adenine 66 (red)
10Nucleotides sheet: thymine 66(green)
10DNA stands, labels, sheets
2Mutant CFTR
2Normal CFTR
2Primer 1
2Primer 2
2Template-Mutant CFTR
2Template-Normal CFTR
10Electrophoresis Bands, labels, sheets
1Mutant CFTR PCR product (22 bp)
1Mutant CFTR RFLP product (10 bp)
1Mutant CFTR RFLP product (14 bp)
1Normal CFTR PCR product (25 bp)
1Normal CFTR RFLP product (25 bp)
60Adhesive strips sized for 4 bp, 30 18 bp, 30 21 bp
30Adhesive strips sized for 22 bp, 3 25 bp
10DNA Polymerase
3Glue sticks, removable
10Bags, resealable
10Taql Restriction enzyme
10Workboards, two-sided
Classroom Planning:
To complete this kit requires one ~50-minute class period